midiprep

midiprep
Slang, denoting a medium scale purification of plasmid from a bacterial culture. Usually used to describe preparations from 10-100ml culture. See also miniprep, maxiprep, megaprep.

Dictionary of molecular biology. 2004.

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  • Plasmid preparation — Plasmid miniprep. 0.8% agarose gel ethidium bromide stained. A plasmid preparation is a method used to extract and purify plasmid DNA. Many methods have been developed to purify plasmid DNA from bacteria. These methods invariably involve three… …   Wikipedia

  • Extraction d'ADN — L extraction de l ADN est une technique permettant d isoler l ADN de cellules ou de tissus. L ADN ainsi extrait peut ensuite être utilisé pour des recherches de biologie moléculaire, telles que le séquençage, la PCR ou le clonage. Il existe… …   Wikipédia en Français

  • Extraction de l'ADN — Extraction d ADN L extraction de l ADN est une technique permettant d isoler l ADN de cellules ou de tissus. L ADN ainsi extrait peut ensuite être utilisé pour des recherches de biologie moléculaire, telles que le séquençage, la PCR ou le clonage …   Wikipédia en Français

  • maxiprep — Slang, denoting a large scale purification of plasmid from a bacterial culture. Usually used to describe preparations from 100 500ml culture. See also miniprep, midiprep, megaprep …   Dictionary of molecular biology

  • megaprep — Slang, denoting a medium scale purification of plasmid from a bacterial culture. Usually used to describe preparations from over 500ml culture. See also miniprep, midiprep, maxiprep …   Dictionary of molecular biology

  • miniprep — Slang, denoting a small scale purification of plasmid from a bacterial culture. Usually used to describe preparations from 1 10ml culture. See also midiprep, maxiprep, megaprep …   Dictionary of molecular biology

  • plasmid prep — Generic term for the isolation of recombinant plasmids from liquid bacterial culture, usually by alkaline/detergent lysis, selective precipitation of other components, and affinity purification of plasmid. As this is the most exciting thing most… …   Dictionary of molecular biology

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